Effects of early undernutrition on kinetic parameters of brain acetylcholinesterase from adult rats

Abstract

The effects of undernutrition during early life on acetylcholinesterase (AChE) specific activity of cerebellum, hippocampus, hypothalamus and striatum were examined in rehabilitated adults rats. Undernourished rats were raised by dams maintained on a restricted food scheme from pup birth to pup weaning (day 31). The offspring were maintained on a restricted food schedule until day 38 when they started to have free access to food until 70-80 days of age. Control rats were raised by dams which had free access to food. The results showed that early malnutrition caused a significant increase in AChE specific activity in cerebellum (about 20 percent), striatum (about 40 percent), and hypothalamus (about 30 percent). No changes were found in the hippocampus. Undernutrition caused a significant increase in Vmax when compared to the control group without changes in Km both in cerebellum and striatum. These results suggest that early undernutrition changes AChE concentration in cerebellum and striatum and does not affect the affinity of the enzyme for the substrate.