The role of raphe and tractus solitarius neuronal structures in the modulation of respiratory pattern in rabbits

Abstract

We studied the effects of MNR stimulation on phrenic (Phr) electroneurogram and external intercostal muscles (EI) electromiogram in spontaneously breathing rabbits. Additionally, experiments were performed before and after lignocaine blockade of nucleus tractus solitarii (NTS) to determine whether the information from MNR is transmitted via NTS neurones. The completeness of the blockade of NTS region was checked by studying the Hering-Breuer reflex. MNR was stimulated at the level 2–7 mm rostral to the obex. Stimulation at the rostral part of this region produced inhibition of phasic inspiratory activity, whereas stimulation in the caudal part elicited tonic activity throughout the respiratory cycle. These effects were more pronounced on EI than on Phr. Responses to MNR stimulation were attenuated after lignocaine blockade, suggesting that the neurones located in NTS take part in the transmission of the modulatory information from the MNR to respiratory motoneurones.